Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Workflow of the method comparison. Metabolic labeling: Labeled and unlabeled cell cultures were mixed at different protein ratios prior to protein digestion and mass spectrometric analysis. Intensity ratios were directly determined from the acquired MS spectra. Label-free comparison: Different total amounts of protein was digested and analyzed. Total ion chromatograms were then aligned and intensity ratios were calculated from different raw files.
