Table I. Nonlinear regression results. Shown are the experimental results obtained from fitting the temporal digest data to their mathematical models.
Peptide | Sequence | kpb | kdb | [SNAT]0c | R2 | kp/kd |
---|---|---|---|---|---|---|
4CL3.262–273 | FDIGTLLGLIEK | 0.344 | 0.140 | 61.1 | 0.9224 | 2.45 |
4CL5.262–273 | FEIGSLLGLIEK | 0.466 | 0.179 | 3.33 | 0.9152 | 2.61 |
C3H3.125–134 | VCTLELFSPK | 0.762 | 0.075 | 16.3 | 0.9605 | 10.1 |
C4H1.255–261 | DYFVDER | 1.436 | 0.003 | 16.8 | 0.9632 | 427 |
C4H2.255–261 | DYFVEER | 1.822 | 0.002 | 5.34 | 0.9451 | 847 |
CAD1.184–198 | GGILGLGGVGHMGVK | 1.011 | 0.184 | 35.0 | 0.8903 | 5.49 |
CAld5H1.426–435 | FLEPGVPDFK | 1.670 | 0.016 | 15.3 | 0.9628 | 102 |
CAld5H2.427–436 | FMKPGVPDFK | 1.976 | 0.046 | 4.37 | 0.9490 | 43.3 |
CAld5H2.L.427–436 | FLKPGVPDFK | 1.881 | 0.020 | 11.0 | 0.9748 | 91.9 |
CCoAOMT1.182–206 | VGGLIGYDNTLWNGSVVAPPDAPMR | 1.647 | 0.330 | 108 | 0.6355 | 5.00 |
CCoAOMT2.182–206 | VGGLIGYDNTLWNGSVVAPADAPMR | 1.631 | 0.682 | 85.9 | 0.7946 | 2.39 |
CCoAOMT3.104–115a | EAYEIGLPFIQK | 1.031 | 0.571 | 36.6 | 0.5861 | 1.81 |
CCoAOMT3.217–232a | VEISQISIGDGVTLCR | 2.505 | 0.963 | 66.2 | 0.6535 | 2.60 |
CCR2.299–308 | DLGFEFTPVK | 2.486 | 0.043 | 12.4 | 0.9438 | 57.2 |
COMT2.51–69 | AGPGAFLSTSEIASHLPTK | 0.880 | 0.071 | 1208 | 0.9734 | 12.4 |
HCT1.338–354 | SALDFLELQPDLSALVR | 1.264 | 0.103 | 20.1 | 0.9387 | 12.2 |
HCT6.338–354 | SALDYLELQPDLSALVR | 1.328 | 0.091 | 11.5 | 0.9517 | 14.7 |
PAL1.238–251a | AAGIDSGFFELQPK | 0.982 | 0.553 | 21.0 | 0.8248 | 1.78 |
PAL1.664–675 | EELGTGLLTGEK | 2.399 | 0.014 | 5.75 | 0.9763 | 172 |
PAL2.661–672 | EELGTILLTGEK | 1.147 | 0.109 | 21.8 | 0.9693 | 10.6 |
PAL3.665–676 | EELGTVLLTGEK | 1.089 | 0.138 | 5.34 | 0.9473 | 7.92 |
PAL4 5.614–622 | IGSFEEELK | 2.335 | 0.005 | 31.2 | 0.9819 | 430 |
PO1.136–149 | DGIVSLGGPHIPLK | 1.470 | 0.024 | 0.549 | 0.8513 | 61.3 |
PO8.113–121 | AFEIIEDLR | 0.701 | 0.019 | 17.3 | 0.9455 | 36.4 |
a Regression performed using digests times ≤4 hours owing to rapid decay of native peptide.
b Units are hr−1.
c Units are fmoles per microgram total protein.