Skip to main content
. 2012 Jun 14;11(9):745–757. doi: 10.1074/mcp.M111.016626

Fig. 3.

Fig. 3.

Effect of kinase inhibitors on cell surface expression of ΔF508-CFTR analyzed by flow cytometry. BHK cells stably expressing ΔF508-CFTR-3HA were placed at (A) 27 °C (positive control) for 48 h, or (B) treated with 10 μm (5Z)-7-oxozeaenol, (C) SU5402, (D) SU6668, or (E) RDEA-119/AR-119/BAY869766, at 37 °C. (F) BHK cells stably expressing WT-CFTR. Flow cytometry was then performed on nonpermeabilized cells following immunostaining for the HA epitope located at the ectodomain of ΔF508-CFTR or WT-CFTR, to quantify the amount of cell-surface CFTR in the analyzed cells. (G) Summary of increase in cell surface expression of ΔF508-CFTR (% change in fluorescence intensity) of the hits analyzed by flow cytometry (two independent experiments, 10,000 live cells per treatment per experiment).