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. 2012 Jun 15;125(12):2954–2964. doi: 10.1242/jcs.101592

Fig. 3.

Fig. 3.

Htz1p is associated with H4 K12ac and H3 K4me3 but not H3 K36me3 in yeast. (A,B) Analysis of H4 K12ac in yeast expressing (A) or lacking (B) HA–Htz1p. (C,D) H3 K4me3 in yeast expressing (C) or lacking (D) HA–Htz1p. (E,F) H3 K36me3 in yeast expressing (E) or lacking (F) HA–Htz1p. Yeast were fixed with methanol:acetic acid and incubated with the indicated fluorescently labeled antibodies and analyzed by FLIM-FRET as described in the Materials and Methods. Left column: Fluorescence lifetime distribution of donor (anti-H4 K12ac–FAM-X, anti-H3 K4me3–Alexa-Fluor-488 or anti-H3 K36me3–Alexa-Fluor-488) in the presence of acceptor (anti-HA–Alexa-Fluor-647). Middle column: FLIM from donor channel. Right column: FLIM from acceptor channel. Scale bars: 10 µm. FLIM scale: 1 nanosecond, blue; 4.5 nanoseconds, red. See also Table 1.