Table 1.
FLIM-FRET analysis of histone modifications in yeast and MDA-MB-468 cells
| Cells | Genotype | Antibody targeta | Lifetime (nanoseconds) | FLIM-FRET efficiency | Distance (nm) |
| Yeast | Donor only | H4 K12ac | 4.01±0.0609 | – | – |
| Donor only | H3 K4me3 | 4.07±0.0533 | – | – | |
| Donor only | H3 K36me3 | 4.10±0.0730 | – | – | |
| HTZ1-HA | H4 K12ac + HA | 3.37±0.0812 | 0.16 | 5.93 | |
| HTZ1-HA | H3 K4me3 + HA | 3.51±0.0465 | 0.14 | 7.58 | |
| HTZ1-HA | H3 K36me3 + HA | 3.92±0.0354 | 0.044 | – | |
| HTZ1 | H4 K12ac + HA | 3.91±0.0329 | 0.025 | – | |
| HTZ1 | H3 K4me3 + HA | 3.96±0.0337 | 0.027 | – | |
| HTZ1 | H3 K36me3 + HA | 3.91±0.0372 | 0.046 | – | |
| MDA-MB-468 | H2A.Z | H3 K4me3 (donor only) | 4.01±0.0312 | – | – |
| H2A.Z | H3 K36me3 (donor only) | 4.05±0.0566 | – | – | |
| H2A.Z | H3 K4me3 + H2A.Z | 2.83±0.106 | 0.29 | 6.50 | |
| H2A.Z | H3 K4me3 + HA | 3.97±0.0793 | 0.0099 | – | |
| H2A.Z | H3 K36me3 + H2A.Z | 3.96±0.0129 | 0.022 | – | |
| H2A.Z | H3 K36me3 + HA | 3.98±0.0258 | 0.017 | – |
a
FAM-X for anti-H4 K12ac antibody; Alexa Fluor 488 for anti-H3 K4me3 and anti-H3 K36me3 antibodies; Alexa Fluor 647 for anti-HA and anti-H2A.Z antibodies. R0 = 5.6 nm for Alexa Fluor 488 and Alexa Fluor 647, and 4.5 nm for FAM-X and Alexa Fluor 647.