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. 2012 Jun 15;125(12):2896–2909. doi: 10.1242/jcs.099275

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 7. — Inhibition of splicing in HeLa cells does not result in NPG formation. Inhibition of splicing in HeLa cells by Spliceostatin A (100 ng/ml for 5 hours using a 100 µg/ml stock in methanol) (A) or a morpholino antisense to the U2 snRNA (B). Methanol (0.1%) or a morpholino sense to T. brucei β-tubulin served as controls. Rck (p54) and SC35 proteins were detected by immunofluorescence, using anti-rck/p54 (Bethyl Laboratories) or anti-SC35 (Sigma) antibodies.