Fig. 5.

PINCH^Q38A-Flag rescued embryos do not exhibit the same decreases in adhesion complex protein stability as null mutant embryos. (A) Stage 16–17 null embryos of the genotypes indicated were collected and Western blots were probed with antibodies raised against β-integrin, ILK, PINCH, RSU-1 and Lamin. (B) Protein levels for the genotypes indicated were quantified and are represented as a fraction of wild-type expression. Bars represent the mean ± range for two replicate experiments. (C) Stage 16–17 embryos were collected for two insertion lines each (2nd and 3rd chromosome) from PINCH^wt-Flag and PINCH^Q38A-Flag rescued stocks. western blots were probed with antibodies raised against PINCH, ILK and Lamin. (D) Levels of PINCH and ILK in PINCH^wt-Flag and PINCH^Q38A-Flag rescued embryos are similar to each other and to wild-type levels. Bars represent the median ± range of transgenic PINCH from three different chromosomal insertions for both PINCH^wt-Flag and PINCH^Q38A-Flag rescued flies.