Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1975 Jul;2(7):1023–1042. doi: 10.1093/nar/2.7.1023

Studies on deoxyribonucleases from Saccharomyces cerevisiae. Characterization of two endonuclease activities with a preference for double-stranded DNA.

R Pinon, E Leney
PMCID: PMC343491  PMID: 239390

Abstract

Two new endonuclease activities, endonuclease B and endonuclease C, obtained from yeast nuclear preparations have been separated and partially characterized. Endonuclease B has a primary requirement for Mn2+ which cannot be replaced by Mg2+ or Ca2+, and makes single-strand scissions in double-stranded DNA. Endonculease C is activated by either Mn2+ or Mg2+, and makes single-strand scissions with Mg2+, while with Mn2+, scissions are made which result in double-strand breaks. Neither enzyme is active on denatured DNA, and both are inhibited by yeast RNA. Both enzymes exhibit pH optima at pH 5.0 and PH 7.2, and leave 5'-phosphoryl termini.

Full text

PDF
1023

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernardi G. Mechanism of action and structure of acid deoxyribonuclease. Adv Enzymol Relat Areas Mol Biol. 1968;31:1–49. doi: 10.1002/9780470122761.ch1. [DOI] [PubMed] [Google Scholar]
  2. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  3. Center M. S., Studier F. W., Richardson C. C. The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A. 1970 Jan;65(1):242–248. doi: 10.1073/pnas.65.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark A. J. Toward a metabolic interpretation of genetic recombination of E. coli and its phages. Annu Rev Microbiol. 1971;25:437–464. doi: 10.1146/annurev.mi.25.100171.002253. [DOI] [PubMed] [Google Scholar]
  5. Cox B. S., Parry J. M. The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutat Res. 1968 Jul-Aug;6(1):37–55. doi: 10.1016/0027-5107(68)90101-2. [DOI] [PubMed] [Google Scholar]
  6. Espejo R. T., Canelo E. S., Sinsheimer R. L. DNA of bacteriophage PM2: a closed circular double-stranded molecule. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164–1168. doi: 10.1073/pnas.63.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Espejo R. T., Canelo E. S. The DNA of bacteriophage PM 2. Ultracentrifugal evidence for a circular structure. Virology. 1969 Mar;37(3):495–498. doi: 10.1016/0042-6822(69)90238-4. [DOI] [PubMed] [Google Scholar]
  8. Esposito M. S., Esposito R. E. The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants. Genetics. 1969 Jan;61(1):79–89. doi: 10.1093/genetics/61.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Franklin R. M., Salditt M., Silbert J. A. Structure and synthesis of a lipid-containing bacteriophage. I. Growth of bacteriophage PM2 and alterations in nucleic acid metabolism in the infected cell. Virology. 1969 Aug;38(4):627–640. doi: 10.1016/0042-6822(69)90182-2. [DOI] [PubMed] [Google Scholar]
  10. GEIDUSCHEK E. P., DANIELS A. A SIMPLE ASSAY FOR DNA ENDONUCLEASES. Anal Biochem. 1965 Apr;11:133–137. doi: 10.1016/0003-2697(65)90052-7. [DOI] [PubMed] [Google Scholar]
  11. Kemper B., Hurwitz J. Studies on T4-induced nucleases. Isolation and characterization of a manganese-activated T4-induced endonuclease. J Biol Chem. 1973 Jan 10;248(1):91–99. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Masamune Y., Fleischman R. A., Richardson C. C. Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid. J Biol Chem. 1971 Apr 25;246(8):2680–2691. [PubMed] [Google Scholar]
  14. Melgar E., Goldthwait D. A. Deoxyribonucleic acid nucleases. II. The effects of metals on the mechanism of action of deoxyribonuclease I. J Biol Chem. 1968 Sep 10;243(17):4409–4416. [PubMed] [Google Scholar]
  15. Piñon R. Characterization of a yeast endonuclease. Biochemistry. 1970 Jul 7;9(14):2839–2845. doi: 10.1021/bi00816a013. [DOI] [PubMed] [Google Scholar]
  16. Radding C. M. Molecular mechanisms in genetic recombination. Annu Rev Genet. 1973;7:87–111. doi: 10.1146/annurev.ge.07.120173.000511. [DOI] [PubMed] [Google Scholar]
  17. Richardson C. C. Enzymes in DNA metabolism. Annu Rev Biochem. 1969;38:795–840. doi: 10.1146/annurev.bi.38.070169.004051. [DOI] [PubMed] [Google Scholar]
  18. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Richardson J. P. Mechanism of ethidium bromide inhibition of RNA polymerase. J Mol Biol. 1973 Aug 25;78(4):703–714. doi: 10.1016/0022-2836(73)90290-8. [DOI] [PubMed] [Google Scholar]
  20. Rodarte-Ramón U. S., Mortimer R. K. Radiation-induced recombination in Saccharomyces: isolation and genetic study of recombination-deficient mutants. Radiat Res. 1972 Jan;49(1):133–147. [PubMed] [Google Scholar]
  21. Roth R., Fogel S. A system selective for yeast mutants deficient in meiotic recombination. Mol Gen Genet. 1971;112(4):295–305. doi: 10.1007/BF00334431. [DOI] [PubMed] [Google Scholar]
  22. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  23. Schulze I. T., Colowick S. P. The modification of yeast hexokinases by proteases and its relationship to the dissociation of hexokinase into subunits. J Biol Chem. 1969 May 10;244(9):2306–2316. [PubMed] [Google Scholar]
  24. Simchen G., Piñon R., Salts Y. Sporulation in Saccharomyces cerevisiae: premeiotic DNA synthesis, readiness and commitment. Exp Cell Res. 1972 Nov;75(1):207–218. doi: 10.1016/0014-4827(72)90538-1. [DOI] [PubMed] [Google Scholar]
  25. Weiss B., Live T. R., Richardson C. C. Enzymatic breakage and joining of deoxyribonucleic acid. V. End group labeling and analysis of deoxyribonucleic acid containing single straned breaks. J Biol Chem. 1968 Sep 10;243(17):4530–4542. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES