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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Curr Protoc Microbiol. 2012 May;CHAPTER:Unit–3D.2.. doi: 10.1002/9780471729259.mc03d02s25
Mating Donor Recipient
Donor Control 50 µl cells -----------------------
Recipient Control ----------------------- 50 µl cells
Experimental 50 µl cells 50 µl cells