Template (your genomic DNA solution 30–120 ng)	1.5 µl
Primer 1 (specific primer, 20 pmol/µl) MarLseq	1.0 µl
Primer 2 (arbitrary primer, 100 pmol/µl) MarTDL2	1.0 µl
10 mM dNTP mix	1.0 µl
10× Tricine Buffer	5.0 µl
Taq polymerase	0.5 µl
20 mM MgCl2 (final concentration in reaction is 2.5 mM)	6.3 µl
ddH2O	33.7 µl
Total Volume	50 µl