Template (your genomic DNA solution 30–120 ng) | 1.5 µl |
Primer 1 (specific primer, 20 pmol/µl) MarLseq | 1.0 µl |
Primer 2 (arbitrary primer, 100 pmol/µl) MarTDL2 | 1.0 µl |
10 mM dNTP mix | 1.0 µl |
10× Tricine Buffer | 5.0 µl |
Taq polymerase | 0.5 µl |
20 mM MgCl2 (final concentration in reaction is 2.5 mM) | 6.3 µl |
ddH2O | 33.7 µl |
Total Volume | 50 µl |