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. Author manuscript; available in PMC: 2012 Sep 6.
Published in final edited form as: Virology. 2011 Jul 23;417(2):268–280. doi: 10.1016/j.virol.2011.06.008

Figure 1.

Figure 1

In vitro phosphorylation of UL44 by UL97 and cdk1/cyclin B. (A) A large-scale phosphorylation reaction was performed with UL44 alone (lane 1) or UL44 incubated with GST-UL97 (lane 2), and the products were resolved by SDS-PAGE and Coomassie-stained. (B) Radiolabeled phosphorylation reactions were performed with either cdk1/cyclin B (cycB) or UL97 and the substrates lamin A, UL44, or UL44ΔC290 (500 nM), and the products were resolved by SDS-PAGE. Reactions were assembled as indicated. The kinase inhibitors used were CGP74514A (cdk1 inhibitor) or maribavir (UL97 inhibitor). The positions of the various proteins are indicated on the right with arrows. (C) A large scale phosphorylation reaction assay was performed with UL44 and cdk1/cycB, and the products were resolved by SDS-PAGE. For all panels, the positions of UL44 and hyperphosphorylated UL44 (UL44-P) are indicated by arrows. The molecular weights of protein markers are indicated on the left.