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. Author manuscript; available in PMC: 2012 Sep 6.
Published in final edited form as: Virology. 2011 Jul 23;417(2):268–280. doi: 10.1016/j.virol.2011.06.008

Figure 5.

Figure 5

Relative phosphorylation levels of UL44 Phos mutants. HFF cells were either mock-infected or infected with parental (AD169rv) or Phos mutant viruses at an MOI of 1 PFU/cell and pulse-labeled for 2 hours with [32P]-orthophosphate at 70 hpi. UL44 immunoprecipitations were prepared from cell lysates harvested at 72 hpi, resolved by SDS-PAGE, transferred to a PVDF membrane. The normalized level of phosphorylation of UL44 from each virus was calculated as the ratio of phosphate incorporation (relative to the AD169rv control) to protein level (relative to the AD169rv control) as described for Fig. 3. The phosphorimage and Western blot of the membrane are shown in Supplementary Figure 3.