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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Neurosci. 2012 Jun;15(6):845–852. doi: 10.1038/nn.3089

FIGURE 5. PKC activation enhances KAR recycling in neurons.

FIGURE 5

a. PMA but not chelerythrine increased the amount of KAR recycling. Scheme illustrates the experimental procedure to selectively analyse surface reinsertion of KARs. Representative images show an increase in surface GluK2 in hippocampal neurones after antibody stripping in the presence of PMA but not chelerythrine. b. Incubation of hippocampal slices in primaquine (1 μM) to inhibit exocytosis from early endosomes prevented the increase in KAR EPSC caused by application of PMA (1 μM). Responses for each cell are normalised to the first 10 minutes. Example traces are taken as the average between 5-10 minutes (black) and 20-25 minutes (grey). Scale bars are 20 pA and 50 ms. Data are plotted as mean ± s.e.m.