Fig. 4.

AG treatment inhibits tumorigenesis in Ikkβ(EE)^IEC/Apc^+/ΔIEC mice. (A) Tumor number and tumor load were determined in 3-mo-old mice of indicated genotype (n > 10 per group); β-catenin⁺ foci were determined in 6-wk-old mice (n > 8). AG mice were treated with 2 g/L of AG in drinking water starting at 4 wk of age. (B) Immunoblot analysis of γ-H2AX in SI villi and crypts of 2-mo-old mice untreated (−) or treated (+) with AG for 2 wk. Quantification of band intensity for crypts is shown. (C) Immunofluorescence analysis with γ-H2AX antibody of frozen sections of 2-mo-old WT and Ikkβ(EE)^IEC mice, untreated or treated with AG for 2 wk.