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. 2012 Aug 13;109(35):14224–14229. doi: 10.1073/pnas.1207461109

Fig. 2.

Fig. 2.

Estrogen is implicated in the hippocampal NO-mediated sex difference in affective behaviors. (A) Fluorescence images of cultured rat hippocampal slices from nNOS KO or WT male mice loaded with DAF-FM DA. (Scale bar, 400 μm.) (B) A hypothesized mechanism underlying the sex difference in depression-like behaviors. (C) Immunoblots showing nNOS levels in E2-treated hippocampal neurons (n = 4). (D) Immunoblots showing nNOS levels in the hippocampal neurons incubated with 10 nM E2 for 24 h or withdrawal (n = 5). (E) Immunoblots showing hippocampal nNOS levels in OVX-female mice treated with E2 or vehicle (n = 4). (F) Immobility time in the TST and FST for OVX-female mice treated with intrahippocampal E2 alone or in combination with 7-NI (n = 13–19). (G and H) Immobility time in the FST (G) and TST (H) of nNOS KO (n = 12–14) and WT (n = 17–18) mice treated with intrahippocampal E2. (I) Immobility time in the TST and FST of OVX-female mice treated with intrahippocampal DPN alone or in combination with C-PTIO or ODQ (n = 14–16). Means ± SEM. *P < 0.05; **P < 0.01, ***P < 0.001. In C, vs. vehicle.