Fig. 1.
Strategy for the detection of telomere fusions using TAR fusion PCR. (A) Fusion junctions were amplified by multiplex primer sets (e.g., primer A, primer B; Table S1) annealing within TAR1 regions adjacent to telomeric DNA sequences. The fusion junctions were detected by Southern blot analysis using a telomeric DNA [TTAGGG]4 probe. (B) Chromosome locations of multiplex PCR primers used for TAR fusion PCR. Black arrows represent primers and the distances (bp) from telomeric DNA starting positions, open arrows denote telomeric DNA, and open rectangles denote genomic regions currently not available in current human genome sequence databases.