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. 2012 Aug 13;109(35):14230–14235. doi: 10.1073/pnas.1206093109

Fig. 5.

Fig. 5.

Fingolimod injection increases ERK1/2 phosphorylation and BDNF levels in cortical neurons. (A) Fingolimod injections (0.1 mg/kg; i.p.) in P30 animals increase phospho-ERK1/2 levels after 30 min as revealed by Western blot analyses of the hippocampus. (B) Fingolimod injections (0.1 mg/kg; i.p.) in P30 animals increase pERK1/2 in neurons 30 min after injection as revealed by immunohistochemistry of the hippocampus. SG, Stratum Granulosum; h, hilus. (C) BDNF mRNA levels are increased in the hippocampus 30 min after fingolimod injection as determined by qPCR. (D–F) BDNF protein levels determined by ELISA in cortex, hippocampus and striatum 48 h after fingolimod injections (0.1 mg/kg; i.p). *P < 0.05; **P < 0.01; ***P < 0.001 (n = 3; mean ± SEM; Student t test). (Scale bar: 20 μm.)