Fig. 2.

Mef2C is required for ECR5-dependent Sost expression, in vivo. ECR5^LacZ transgenic mice that were also mutant for Mef2C did not express LacZ in the femur (B) or calvaria (F) relative to the ECR5^LacZ controls (A and E). Similar results were obtained in Mef2C^cKO; Col1-Cre; Sost^KO double knockout animals, where LacZ expression was dramatically reduced in the femur (D) and calvaria (H) relative to Sost^LacZ controls (C and G).