Table 1.

Bone phenotying based on histomorphometric indices in the cancellous bone compartment of the distal femurs of 6-mo-old ECR5^KO, Sost^KO, and Mef2C^cKO;Col1-Cre male mice compared with WT controls

Histomorphometric index	WT	ECR5KO	% change	SostKO	% change	WT	Mef2CcKO	% change
BV/TV	7.79 ± 4.02	15.894 ± 5.833*	+104	28.45 ± 2.79*	+265	0.127 ± 0.02	0.201 ± 0.05*	+58
BS/BV	70.90 ± 20.54	48.86 ± 12.54*	−31	39.89 ± 4.82*	−44	56.72 ± 9.92	40.39 ± 7.68*	−29
Tb.Dm (Plate)	30.33 ± 8.39	43.82 ± 12.57*	+44	50.85 ± 6.77*	+68	—	—	—
Tb.N (Plate)	2.53 ± 1.16	4.26 ± 1.67*	+68	5.63 ± 0.94*	+122	—	—	—
Tb.Sp (Plate)	438.3 ± 211.7	222.6 ± 83.80*	−49	131.81 ± 29.82*	−70	—	—	—
Tb.Dm (Rod)	—	—	—	—	—	0.072 ± 0.010	0.097 ± 0.02*	+35
Tb.N (Rod)	—	—	—	—	—	5.652 ± 0.87	5.299 ± 0.81	−6
Tb.Sp (Rod)	—	—	—	—	—	0.108 ± 0.02	0.086 ± 0.01*	−20
Ob.S	2.62 ± 1.11	5.956 ± 1.89*	+127	6.143 ± 1.68*	+134	1.402 ± 1.55	3.326 ± 4.40	+137
Ob.S/BS	14.42 ± 3.93	17.730 ± 6.54*	+23	17.16 ± 2.62	+19	0.130 ± 0.15	0.046 ± 0.02	−65
Oc.S	1.64 ± 0.76	1.404 ± 0.364	−14	1.623 ± 0.38	−1	1.743 ± 1.25	1.219 ± 0.73	−30
Oc.S/BS	8.92 ± 3.29	4.426 ± 1.87*	−50	4.608 ± 0.89*	−48	0.067 ± 0.04	0.049 ± 0.02	−27
MAR (μm/d)	2.06 ± 0.36	2.634 ± 0.324*	+28	2.938 ± 0.165*	+42	0.981 ± 0.15	1.368 ± 0.33*	+39
BFR/BS	0.79 ± 0.19	1.188 ± 0.40*	+50	1.035 ± 0.28	+31	0.073 ± 0.02	0.108 ± 0.13	+48
BFR/BV	56.29 ± 23.36	59.89 ± 32.58	+6	40.062 ± 7.96	−29	3.027 ± 1.46	2.829 ± 1.66	−7
BFR/TV	3.87 ± 1.41	8.371 ± 2.84*	+116	11.46 ± 2.57*	+196	0.306 ± 0.12	0.736 ± 0.22*	+141

Data represent mean ± SD for parameters measured. Group size n = 5–10. *P < 0.05. BFR, bone formation rate; BS, bone surface; BV, bone volume; MAR, mineral apposition rate; Ob.S, osteoblast surface; Oc.S, osteoclast surface; Tb.Dm, trabecular diameter; Tb.N, trabecular number; Tb.Sp, trabecular separation; TV, total volume; —, no measurements were conducted.