Fig. 2.

STORM images of four membrane organelles/structures in live cells. Conventional (i) and STORM (ii) images of (A) the plasma membrane labeled with DiI in a hippocampal neuron, (B) mitochondria labeled with MitoTracker Red in a BS-C-1 cell, (C) the ER labeled with ER-Tracker Red in a BS-C-1 cell, and (D) lysosomes labeled with LysoTracker Red in a BS-C-1 cell. The conventional fluorescence images were taken immediately before STORM imaging with a low excitation intensity to avoid switching off the dyes appreciably. The STORM images were acquired in 15 sec (A), 10 sec (B and C) and 1 sec (D). Arrowheads in A indicate spine necks along with their measured widths. Scale bars, 1 μm.