Fig. 4.

Lysosomal storage in astrocytes induces cortical neuron degeneration in an ex vivo coculture assay. (A and B) (Left) MAP2 (green) and GFAP (red) immunostaining of neurons and astrocytes, respectively, untreated (A) or treated with glutamate 0.1 mM for 24 h (B). (Right) Histograms represent quantification of MAP2⁺ neurons after 12 d in culture (A) and quantification of MAP2⁺ neurons after glutamate stimulation relative to the same sample without stimulation (B). Data represent mean ± SEM from three independent coculture experiments. *P ≤ 0.05, Student’s t test. (Scale bars: 20 μm.)