EPNs differ in their virulence toward potential hosts. Graphs show the virulence of each nematode toward the panel of potential hosts. Values for “death” represent the fraction of hosts that died within 48 h following exposure to nematodes. Values for “growth,” “reproduction,” and “emergence” represent the fraction of dead hosts that supported nematode growth, reproduction, and emergence, respectively. The frequency of death following exposure to nematodes was scored for all potential hosts; growth, reproduction, and emergence were scored only when host killing was observed at statistically significant levels. Each virulence assay consisted of a single potential host and 100 IJs. n = 20–50 assays for all invertebrates except flatheaded borers; n = 8–12 assays for flatheaded borers because of the limited availability of these insects. For each EPN–host combination, statistical significance was determined relative to an uninfected control using a χ2 test. Mean values for death, growth, reproduction, and emergence are given in Dataset S6.