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. 2012 Aug 13;109(35):14019–14023. doi: 10.1073/pnas.1212545109

Fig. 2.

Fig. 2.

MK1 promotes megakaryocyte differentiation at the expense of the granulocyte and monocyte lineages. (A) Phenotype of the progeny of 5,000 mPB CD34+ cells cultured for 10 d in the presence or absence of MK1 (0.75 μM). Megakaryocytes (CD41), stem and progenitor cells (CD41−CD34+), and single lineage progenitors/mature cells (CD41−CD34−) are gated. (B) Absolute number of megakaryocytes (white, P < 0.01), stem and progenitor cells (black), and single lineage progenitors/mature cells (gray) in A. (C) Phenotype of the stem and progenitor cells (CD41−CD34+) gated in A: CMPs (CD41−CD34+CD110−CD45RA−), GMPs (CD41−CD34+CD110−CD45RA+), and MEPs (CD41−CD34+CD110+CD45RA−) are gated. (D) Absolute numbers of CMPs (white), GMPs (gray), and MEPs (black) in C (P < 0.01). (E) Phenotype of single lineage progenitors/mature cells (CD41−CD34−) gated in A: Granulocyte or monocyte progenitors (CD41−CD34−CD110−CD45RA+), erythroid progenitors (CD41−CD34−CD110+CD45RA−), and mature cells (CD41−CD34−CD110−CD45RA−) are gated. (F) Absolute number of mature cells (white), granulocyte or monocyte progenitors (gray), and erythroid progenitors (black) in E (P < 0.02). Data are representative of at least three independent experiments.