Table 1. Cryptosporidium species and genotypes in wastewater samples from four cities in China during 2006–2009.

Species/genotype or gp60 subtype	Ref No.	No. of positive samples (No. of positive PCR products)a
		Shanghaib	Nanjing	Qingdao	Wuhan
Humans
C. hominis	EF570922 or AY204230	59 (78)	6 (9)	1 (1)	19 (21)
IaA18R4	FJ153246	3 (3)
IaA19R4	FJ153245	3 (3)
IaA27R3	AF164502		1 (1)
IbA19G2	FJ707312	5 (6)c	1 (1)
IbA20G2	FJ707313	3 (3)
IbA21G2	FJ153239	22 (35)c
IdA14	GU214350	2 (2)
IdA16	HQ149034	2 (3)	1 (1)		1 (1)d
IeA11G3T3	GU214354				1 (1)
IeA12G3T3	FJ153242	6 (7)	1 (1)	1 (1)	9 (12)d
IfA20G1	FJ153243	1 (1)
IfA22G1	FJ153244	4 (5)
Cryptosporidium W13314	HM191258	1 (1)
Livestock and companion animals
C. parvum	AF093490	1 (1)		2 (3)
IIdA19G1	HQ009809			2 (3)
C. andersoni	FJ463187				4 (5)
C. canis	EU754833			1 (2)	1 (1)
C. suis	AF115377 or GQ345008	1 (2)	7 (7)	6 (7)	17 (20)
Pig genotype II	AB449825		1 (1)		5 (5)
C. cuniculus	EU437413		3 (3)		2 (2)
VaA31	FJ262734		1 (1)
Birds
C. meleagridis	AF112574	7 (7)	2 (2)	7 (7)	6 (6)
C. baileyi	AF093495 or EU717825	3 (3)	6 (6)	2 (2)	4 (4)
Avian genotype III	HM116386	1 (1)
Rodents
C. muris	AF093498	1 (1)	18 (24)	2 (2)e	10 (10)
Rat genotype I	AY268584	1 (1)	1 (1)	2 (2)	1 (2)
Rat genotype IV	AY737585, AY737580 or AY737584			8 (8)	2 (2)
Totalf		63/90 (70.0%)	32/87 (36.8%)	75/109 (68.8%)	47/100 (47.0%)

^aAll data on specific genotypes and subtypes are based on sequence analysis.

^b Cryptosporidium data from Shanghai wastewater samples were previously reported in [16].

^cConcurrent presence of IbA19G2 and IbA21G2 was found in one sample.

^dConcurrent presence of IdA16 and IeA12G3T3 was found in one sample.

^eA total of 60 (109) were positive for C. muris by SspI and DdeI RFLP analysis.

^fNo. of positive samples/No. of samples (positivity in percentage) based on PCR analysis of the SSU rRNA gene.