Table 2. Giardia duodenalis assemblages and genotypes in wastewater samples of four cities in China during 2006–2009.
| Genotype or subtype | Ref No. | No. of positive samples (No. of positive PCR products)a | |||
| Shanghai | Nanjing | Qingdao | Wuhan | ||
| Humans | |||||
| A2 | U57897 | 51 (105) | 38 (76) | 97 (180) | 57 (86) |
| Mammals (including humans) | |||||
| B1 | L02116 | 1 (1) | |||
| B2 | 17238-1 | 1 (1) | |||
| B3 | 17257-2 | 1 (1) | |||
| B4 | 17239-1 | 1 (1) | |||
| B5 | 19068-2 | 1 (1) | |||
| A2+B1b | U57897, L02116 | 1 (1) | |||
| A2+B2b | 17238-1 | 2 (5) | |||
| A2+B3b | 17257-2 | 3 (6) | |||
| A2+B4b | 17239-1 | 1 (2) | |||
| A2+B6b | 17219-2 | 1 (2) | |||
| A2+B7b | 18930-2 | 1 (2) | |||
| A2+B8b | 17231-2 | 1 (5) | |||
| A2+B9b | 18933-2 | 1 (2) | |||
| A2+B10b | 17252-1 | 1 (4) | |||
| A2+B11b | 18972-2 | 1 (2) | |||
| A2+B12b | 17259-1 | 1 (2) | |||
| A2+B13b | 19088-1 | 1 (2) | |||
| A2+B14b | 17241-2 | 1 (2) | |||
| A2+B15b | 19121-2 | 1 (2) | |||
| A2+Bc | 30 (38) | 6 (10) | |||
| B2+B16 | 17238-1, 19108-2 | 1 (2) | |||
| Totald | 85/90 (94.5%) | 59/87 (67.8%) | 106/109 (97.2%) | 69/100 (69.0%) | |
a
All data on specific genotypes and subtypes are based on sequence analysis.
b
Different sequencing results in different PCR replicate analyses of each sample. B2–B16 are new subtypes identified in this study.
c
Based on the occurrence of underlying peaks in DNA sequencing trace files; the subtype identity of assemblage B could not be established under such a circumstance.
d
No. of positive samples/No. of samples (positivity in percentage) based on PCR analysis of the tpi gene.