Table 2. Minimum inhibitory concentrations (MIC90) towards GBS strains mutated in various cell surface components.
WT | ΔdltA | ΔmprF | Δlgt Δlsp | ΔcpsD | ΔsrtA | |
K9L6 | 25 | 25 | ND | ND | ND | ND |
5D-K6L9 | 4.4 | 2.2 | 4.4 | 4.4 | 4.4 | 4.4 |
K6L9 | 35.5 | 17.7 | 17.7 | 17.7 | 35.5 | 35.5 |
K5L7 | 25 | 25 | ND | ND | ND | ND |
C8-K5L7 | 3 | 3 | ND | ND | ND | ND |
LL-37 | >14.2 | 3.6 | >14.2 | >14.2 | >14.2 | >14.2 |
Magainin 2 | >25.5 | 6.4 | >25.5 | >25.5 | >25.5 | >25.5 |
Polymyxin B | 92.4 | 5.8 | 92.4 | 92.4 | 92.4 | 92.4 |
Colistin | >221.6 | 13.9 | >221.6 | >221.6 | >221.6 | >221.6 |
The MIC (µM) of each antimicrobial peptide is an average of triplicate measurements performed by a dilution method in 96-well polypropylene microplate. Plates were incubated overnight at 37°C and were then read at OD600 nm for bacterial growth. The MICs90 was considered to be the peptide concentration that inhibited growth of 90% of the tested strains.