Table 3. Phenotypes of the cap1 mutant in growth, conidiation, and plant infection.
Strain | Growth rate (mm/day)a | Conidiation (×105 spores/plate) | Appressoria/germ tubeb (%) | Lesions/5 cm leaf tipc | Appressorium penetrationd (%) |
Ku80 (wt) | 3.3±0.1 | 296.3±28.8 | 96.2±1.8 A* | 51.3±4.5 A | 68.4±5.4 A |
HC83 (Δcap1) | 1.7±0.2 | 112.1±13.4 | 51.3±1.7 C | 9.4±2.1 D | 15.2±3.2 D |
CH07 (Δcap1/CAP1) | 3.3±0.1 | 310.5±21.2 | 95.0±2.0 A | 47.3±5.2 A | 62.5±4.8 A |
HC10 (CAP1 ΔAB) | 2.7±0.1 | 259.7±5.9 | 91.5±1.4 A | 31.5±1.3 B | 49.6±5.1 B |
XY111 (CAP1 ΔACB) | 1.8±0.1 | 153.6±11.6 | 59.6±2.7 C | 11.5±2.9 D | NA |
XY244 (CAP1 ΔP2) | 3.1±0.2 | 307.3±25.3 | 69.0±1.2 C | 17.3±4.3 C | 33.2±1.9 C |
Growth rate and conidiation were measured on complete medium (CM). Mean and standard deviation were calculated with results from three replicates.
Appressorium formation is expressed as the percentage of germ tubes producing appressorium.
Lesion formation was examined on infected rice leaves 7 days post-inoculation.
Means and SD values were calculated from at least three independent experiments.
Appressorium penetration was assayed on onion epidermis at 48 h after inoculation.
The same letter indicated there was no significant difference. Different letters were used to mark statistically significant differences (P = 0.05).