Table 1. Oligonucleotide primers and PCR conditions of rat in quantitative real-time PCR.

Genes	Forward primer	Reverse primer	Product (bp)	Annealing
11β-HSD-1	GGAGCCCATGTGGTATTGA	AGTGCCGGCAATGTAGTGA	105	61°C, 20 s
11β-HSD-2	AGGGGACGTATTGTGACCG	GCTGGATGATGCTGACCTTG	154	63°C, 20 s
GR	CACCCATGATCCTGTCAGTG	AAAGCCTCCCTCTGCTAACC	156	60°C, 20 s
CRH	TGGATCTCACCTTCCACCTTCTG	CCGATAATCTCCATCAGTTTCCTG	103	62°C, 20 s
StAR	GGGAGATGCCTGAGCAAAGC	GCTGGCGAACTCTATCTGGGT	188	65°C, 20 s
P450scc	GCTGCCTGGGATGTGATTTTC	GATGTTGGCCTGGATGTTCTTG	156	63°C, 20 s
GAPDH	TAAAGAACAGGCTCTTAGCACA	AGTCTTGGAAATGGATTGTCTC	107	59°C, 15 s

11β-HSD-1, 11β-hydroxysteroid dehydrogenase 1; 11β-HSD-2, 11β-hydroxysteroid dehydrogenase 2; GR, glucocorticoid receptor; CRH, corticotropin releasing hormone; StAR, steroid acute regulatory protein; P450scc, cholesterol side chain cleavage enzyme; GAPDH, glyceraldehyde phosphate dehydrogenase.