Table 1. Oligonucleotide primers and PCR conditions of rat in quantitative real-time PCR.
Genes | Forward primer | Reverse primer | Product (bp) | Annealing |
11β-HSD-1 | GGAGCCCATGTGGTATTGA | AGTGCCGGCAATGTAGTGA | 105 | 61°C, 20 s |
11β-HSD-2 | AGGGGACGTATTGTGACCG | GCTGGATGATGCTGACCTTG | 154 | 63°C, 20 s |
GR | CACCCATGATCCTGTCAGTG | AAAGCCTCCCTCTGCTAACC | 156 | 60°C, 20 s |
CRH | TGGATCTCACCTTCCACCTTCTG | CCGATAATCTCCATCAGTTTCCTG | 103 | 62°C, 20 s |
StAR | GGGAGATGCCTGAGCAAAGC | GCTGGCGAACTCTATCTGGGT | 188 | 65°C, 20 s |
P450scc | GCTGCCTGGGATGTGATTTTC | GATGTTGGCCTGGATGTTCTTG | 156 | 63°C, 20 s |
GAPDH | TAAAGAACAGGCTCTTAGCACA | AGTCTTGGAAATGGATTGTCTC | 107 | 59°C, 15 s |
11β-HSD-1, 11β-hydroxysteroid dehydrogenase 1; 11β-HSD-2, 11β-hydroxysteroid dehydrogenase 2; GR, glucocorticoid receptor; CRH, corticotropin releasing hormone; StAR, steroid acute regulatory protein; P450scc, cholesterol side chain cleavage enzyme; GAPDH, glyceraldehyde phosphate dehydrogenase.