. 2012 Sep 6;7(9):e44451. doi: 10.1371/journal.pone.0044451

Table 1. Summary of the trials performed to determine the basis of protection following bacterial re-exposure in rag1^−/− mutant zebrafish.

Trial	Primary	Treatment; Objective; # of experiments; tanks/treatment; fish/tank	Secondary
1	E. ictaluri RE33 10⁴	4 week interval; Determine if protection occurs; 3 exps; 3 tanks/trt; 24 fish/tank	E. ictaluri 10⁴
2	E. ictaluri RE33 10⁴	8 week interval; Rule out temporarily heightened primary effects; 2 exps; 8 tanks/trt;10 fish/tank	E. ictaluri 10⁴
3	E. ictaluri RE33 10⁴	4 week interval with antibiotic administered; Rule out persistent pathogen presence;2 exps; 8 tanks/trt; 10 fish/tank	E. ictaluri 10⁴
4	E. ictaluri RE33 10⁴	4 week interval with homologous or heterologous secondary; Determine specificity;1 exp; 4 tanks/trt; 15 fish/tank	E. ictaluri 10⁴ Y. ruckeri 10⁶ E. tarda 10²
5	E. ictaluri RE33 10⁴	4 week interval with adoptive cell transfers; determine if protection provided bya leukocyte population; 1 exp; 7 tanks/trt; 15 fish/tank	E. ictaluri 10⁴

Table 1. Summary of the trials performed to determine the basis of protection following bacterial re-exposure in rag1−/− mutant zebrafish.