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. 2012 Sep 6;7(9):e44451. doi: 10.1371/journal.pone.0044451

Table 1. Summary of the trials performed to determine the basis of protection following bacterial re-exposure in rag1−/− mutant zebrafish.

Trial Primary Treatment; Objective; # of experiments; tanks/treatment; fish/tank Secondary
1 E. ictaluri RE33 104 4 week interval; Determine if protection occurs; 3 exps; 3 tanks/trt; 24 fish/tank E. ictaluri 104
2 E. ictaluri RE33 104 8 week interval; Rule out temporarily heightened primary effects; 2 exps; 8 tanks/trt;10 fish/tank E. ictaluri 104
3 E. ictaluri RE33 104 4 week interval with antibiotic administered; Rule out persistent pathogen presence;2 exps; 8 tanks/trt; 10 fish/tank E. ictaluri 104
4 E. ictaluri RE33 104 4 week interval with homologous or heterologous secondary; Determine specificity;1 exp; 4 tanks/trt; 15 fish/tank E. ictaluri 104 Y. ruckeri 106 E. tarda 102
5 E. ictaluri RE33 104 4 week interval with adoptive cell transfers; determine if protection provided bya leukocyte population; 1 exp; 7 tanks/trt; 15 fish/tank E. ictaluri 104