Table 1. miRNA and anti-miR inhibitor sequences.
Type | Name | Sequence |
miRNAa | miR-16+ | UAGCAGCACGUAAAUAUUGGCG |
miR-16− | CCAAUAUUUACGUGCUGUUAUU | |
miR-16 scramble+ | AUCCACCACGUAAAUAUUGGCG | |
miR-16 scramble- | CCAAUAUUUACGUGGUGGAUCG | |
Anti-miRb | Anti-miR-16 | CˆGCCˆAATˆATTˆTACˆGTGˆCTGˆCTA |
Anti-miR-16 scramble | CˆGCCˆAATˆATTˆTACˆGTGˆGTGˆGAT |
Synthetic miRNA were designed to mimic the endogenous miRNA, with ‘+’ indicating the mature miRNA strand, and ‘–’ indicating the passenger strand.
nti-miR inhibitor oligonucleotides were designed complementary to the mature miRNA, with LNA-modified nucleotides preceded by a ‘∧’ symbol.