Figure 2. Thioredoxin activity.

The reduction of the insulin alfa-chain was monitored at 650 nm in bacteria treated with 260 mM Fe³⁺, 4 mM diamide, or 1 mM H₂O₂, at 30 (A) or 60 minutes (B) of stress exposure. The reaction was performed as described in materials and methods after 15 min of incubation. Activity in the control reaction corresponds to 100%. Data represents the average of two independent trials (lines on top of bars indicate values range). The negative control (mixture without protein) did not reduced insulin alfa-chain at 15 min.