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. 2012 Sep 6;7(9):e44661. doi: 10.1371/journal.pone.0044661

Figure 2. Determination of the transcription start site of the human KIAA1199. A).

Figure 2

5′ primer extension analysis: Mapping of the KIAA1199 mRNA start site by non-radioactive 5′ primer extension analysis was performed using the reverse primer located between +254 and +234 in the first exon (shown by underlie arrow in Figure 2D). B) Identification of the transcription start site(s) of the KIAA1199 mRNA: 5′ RLM-RACE was performed followed by DNA sequencing analysis. DNA sequencing chromatogram represents a longest transcript and percentages of the alternative transcription start sites are given. C) CAGE data analysis: One major transcription start site was identified in the CAGE analysis viewer (CTSS:Cage Transcription Start Site). D) Nucleotide sequence of the 5′-flanking region of the KIAA1199: +1 was given to the transcription start site of KIAA1199. According to the +1, TATA-box was identified between −31 and −27; GC-box was identified between −248 and −243. Start site of CpG island was shown with gray box in the upstream region of promoter and nucleotides in the CpG island are shown by italics.