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. 2012 Sep 6;7(9):e44523. doi: 10.1371/journal.pone.0044523

Figure 11. The upper respiratory tract had a greater increase in the number of mycoplasma-specific antibody-forming cells (AFC) than did the lower respiratory tract.

Figure 11

ELISpot assays were performed on tissue collected at necropsy (14 days post infection except for one calf that was euthanized at 10 days post infection) from control calves (N = 5; white bars) and from calves inoculated by oral (N = 4; black bars) or transtracheal (N = 3; hatched bars) routes. Tissues from other calves in the study could not be analyzed due to low cell recovery. Cells were collected from Medial retropharyngeal lymph nodes (MRPLN), Lateral retropharyngeal lymph nodes (LRPLN), Tracheobronchial lymph nodes (TBLN) and Lung. The data for lymph nodes are represented as the mean ± SD of the total number of cells within that tissue. Data for the lungs are represented as the mean (± SD) of the number of cells/g of tissue. abcSuperscript letters indicate significant (P<0.05) differences in class-specific responses between groups.