Figure 4. IFN-γ-induction induces immunoproteasome and rVV-NY-ESO-1 infection significantly increases NY-ESO-1 expression in tumor cells.

Tumor cell line, SK-MEL-8, was treated with either IFN-γ for 48 hrs or 5-aza-dC for 72 hrs. The cells were then stained for their surface class I expression (A). The treated cells were also infected with rVV-NY-ESO-1, or rVV-GFP as a control, for 4 hours. The infected and uninfected cells were then lysed immediately for Western analysis (B). Similar data were obtained from three similar experiments. Note, β-actin was reblotted after stripping the previously bound anti-NY-ESO-1 antibody.