Figure 1. Screen of small molecule library for AHR agonists.

A. A collection of 4160 compounds was screened for the induction of the DRE-driven luciferase in the human hepatoma 101L cell line. In 384-well plates, 100 µL media containing 70% confluent 101L cells was incubated with 10 µM of each test compound (1% v/v DMSO) for 24 hours. Dotted line indicates 3-fold induction. B. Screen for agonists of the AHR^d. The AHRd-15 cell line was treated with 1 µM of the 98 compounds identified from the primary screen, 2 nM TCDD or DMSO and EROD activity was determined. Dashed line indicates 5-fold induction.