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. 2012 Sep 6;7(9):e44724. doi: 10.1371/journal.pone.0044724

Table 1. Table 1 summarizes the solvents used for the stem solutions, the concentration of the stem solutions, the concentration of the working solutions, the volume injected, the concentration in the vitreous body based on an estimated volume of 50 µl [50] up to 100 µl (own measurements in older animals (>400 g), and the number of tests performed.

substance solvent stem solution working solution volume concentration in vitreous tests (effective)
bumetanide ethanol 100mM 300µM 10µl 30–60µM 21 (17)
furosemide acetone 25mM 250µM 10µl 25–50µM 8 (7)
APB 0.9% NaCl 55mM 0.4–10mM 5–10µl 40–200µM 25 (22)
PTX 0.1M PBS 2.5mM 1mM 5–10µl 100–200µM 10 (10)

Numbers in parentheses give the number of tests where drug administration resulted in changes of the neuronal activity. APB: 2-amino-4-phosphonobutyrat; PTX: picrotoxin; PBS: phosphate buffered saline.