Table 1. Table 1 summarizes the solvents used for the stem solutions, the concentration of the stem solutions, the concentration of the working solutions, the volume injected, the concentration in the vitreous body based on an estimated volume of 50 µl [50] up to 100 µl (own measurements in older animals (>400 g), and the number of tests performed.
substance | solvent | stem solution | working solution | volume | concentration in vitreous | tests (effective) |
bumetanide | ethanol | 100mM | 300µM | 10µl | 30–60µM | 21 (17) |
furosemide | acetone | 25mM | 250µM | 10µl | 25–50µM | 8 (7) |
APB | 0.9% NaCl | 55mM | 0.4–10mM | 5–10µl | 40–200µM | 25 (22) |
PTX | 0.1M PBS | 2.5mM | 1mM | 5–10µl | 100–200µM | 10 (10) |
Numbers in parentheses give the number of tests where drug administration resulted in changes of the neuronal activity. APB: 2-amino-4-phosphonobutyrat; PTX: picrotoxin; PBS: phosphate buffered saline.