Table 1. Oligonucleotides primer sequences for PCR and probe preparations.

Targeted genomic Island		Sequence (5′–3′)	Amplicon (bp)	Annealing temperature
G1	F	GCACTCAAAGGGGCTAAAG	1046	55°C
	R	GAAGATAATACGGCGGAATACAAT
G2	F	TCTAAATTCATCGGAGTA	570	48°C
	R	TTTTGAGGGTTATATGAATGTC
G6	F	GTAAAAGCGTGCGTGATGGTATGG	1334	63°C
	R	CGCTGTTTGTGCCGTTGCTAAG
G8	F	ATTGGGTTATATTTTTCTGTC	814	50°C
	R	AATCCCTTTGCTACCATCA
G9	F	TAACTTCAACAATAGGTCGTCCAG	444	52°C
	R	TATCTTCTATTTTAACATCTAC
G10	F	ACGCGCTTTAATTGTTTGGTAGA	1603	58°C
	R	TTTTTGGGTGATATTGTGCTTTAG
G11	F	CAAAGATTATGGCTACCTA	616	50°C
	R	GTTATATTTTCTTACACTCTCC

Note: Product sizes are based on 6P18H1 genome. F – forward primer; R – reverse primer.