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. 2012 Sep 6;7(9):e43730. doi: 10.1371/journal.pone.0043730

Figure 6. ICS analyse of HBV PreS1- or S-specific CD8+ cells producing interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α) and interleukin-4 (IL-4) induced by heterologous rTTV and recombinant protein prime/boost immunization.

Figure 6

Splenocytes from four mice per group were isolated 14 days after last immunization. The splenocytes were exposed to HBV PreS1 peptide(S1–9) or S peptides pool and cytokine production was measured by monoclonal antibody staining and flow cytometric analysis. (a) Flow cytometer plot of results obtained from one representative individual mouse from each group. (b), Average (± SEM) of the percentage of IFN-γ-producing, TNF-α-producing and IL-4-producing CD8+ T cells obtained from four mice per group following stimulation with HBV PreS1 peptide (S1–9) or S peptides pool. Compared with RVJSS1 prime/HBSS1 boost, mice receiving HBSS1+Al(OH)3 prime/RVJSS1 boost generated markedly higher PreS1-specific CD8 T cell responses for IFN-γ(P<0.05) and S- specific CD8 T cell responses for TNF-α(P<0.05), mice receiving HBSS1 prime/RVJSS1 boost generated markedly higher PreS1- and S-specific CD8 T cell responses for TNF-α(P<0.05).