Table 1. Total antibody positivity rate after single or double immunization.

Group	Vaccine or reagent		Anti-S		Anti-PreS1
	Prime	Boost	Prime	Boost	Prime	Boost
1	NS	NS	0	0	0	0
2	RVJ	RVJ	0	0	0	0
3	RVJSS1	HBSS1	2/8	6/8	6/8	8/8
4	HBSS1	RVJSS1	5/8	7/8	8/8	8/8
5	HBSS1+Al(OH)3	RVJSS1	8/8	8/8	8/8	8/8

Note: Mice were randomly assigned to five groups (8/group). Except for the NS and RVJ mock groups, mice received different prime–boost vaccination regimens. Sera were collected 2 weeks after each immunization, and HBV specific antibody was detected by ELISA. Seroconversion is indicated in the table.

NS: Normal saline.

RVJ: recombinant vaccinia virus.