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. 2012 Sep 5;87(3):511–517. doi: 10.4269/ajtmh.2012.11-0708

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Figure 2. — Detection of Lutzomyia 12S ribosomal DNA and of Leishmania kinetoplast DNA by polymerase chain reaction (PCR) of pools of field-collected Lutzomyia sand flies. A, Agarose gel electrophoresis of 12S ribosomal DNA PCR products in a representative subset of pools. Lane 1, 100-basepair (bp) ladder; lanes 2–8, DNA from Lutzomyia pools; lane 9, positive control of male Lutzomyia spp.; lane 10, blank. B, Agarose gel electrophoresis of kinetoplast DNA PCR products. Lane 1, 100-bp ladder; lanes 2–8, positive Lutzomyia pools; lane 9, positive control of L. (V.) braziliensis strain MHOM/BR/84/LTB300; lane 10, negative control of male Lutzomyia spp.; lane 11, blank.