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. 2012 Sep 6;7(9):e44705. doi: 10.1371/journal.pone.0044705

Figure 7. Nd1-L may cooperate with KRIT1 in modulating the expression levels of SOD2.

Figure 7

KRIT1-null (Krit1-) and KRIT1-expressing (Krit1+) MEF cells grown under standard conditions were either mock transfected (−) or transfected with Flag-Nd1-L cDNA (+), and cell lysates were analyzed by Western blot. The expression levels of Flag-Nd1-L were assessed with an antibody against the Flag tag, whereas SOD2 and KRIT1 were detected with specific monoclonal and polyclonal antibodies, respectively. Tubulin served as loading control. Notice that the expression of Flag-Nd1-L induces an upregulation of SOD2 protein levels in KRIT1-expressing but not KRIT1-null MEF cells.