Figure 1. Correlation between the ability of different DBL4ε anti-sera to react with native VAR2CSA expressed on infected erythrocytes (IE) and their ability to inhibit the binding of VAR2CSA-expressing FCR3 IE to CSA.

(A) Anti-DBL4ε sera (n = 75) from rats were tested for reactivity in FACS and for ability to inhibit parasite binding to CSA in a static binding assay. (B) Comparison of anti-VAR2CSA antibody levels in sera which inhibited binding (N = 15) and sera without anti-binding activity (N = 9). Antibody mean titre was determined as EC50 using GraphPad Prism5.