Figure 4. Effects of α-tomatine on the mitochondrial membrane potential in both HL60 and K562 cell lines.

The mitochondrial membrane potential was quantitated by flow cytometric analysis with rhodamine 123. The (A) HL60 and (B) K562 cell lines were treated with 10 µM rhodamine 123 and incubated at 37°C for 30 min in the presence of 5 µM α-tomatine. The horizontal axis shows the relative fluorescence intensity, and the vertical axis indicates the cell number. The green curve indicates the control. The blue curve indicates the α-tomatine-treated cells. A shift from the green curve to the blue curve indicates a loss of mitochondrial membrane potential. Data are expressed from at least three separate determinations.