Table 2. Mutants from the tetO7 promoter replacement collection that exhibited a significantly more oxidized cytosol than wild type after treatment with doxycycline.
Gene | Function | E GSH±SD (−mV) |
Wild type | 322 | |
tet-O7-STT4 | Phosphotidylinositol-4-kinase | 272±6 |
tet-O7-SRP14 | Signal recognition particle complex subunit | 282±16 |
tet-O7-yor146w | Dubious opening reading frame | 284±6 |
tet-O7-CDC34 | Ubiquitin-conjugating enzyme | 291±7 |
tet-O7-YPT1 | Rab family GTPase | 298±14 |
tet-O7-SPC98 | Component of the microtubule-nucleating Tub4p complex | 298±3 |
tet-O7-SAH1 | S-adenosyl-L-homocysteine hydrolase | 298±6 |
tet-O7-SEC53 | Phosphomannomutase | 299±5 |
tet-O7-RIB5 | Riboflavin synthase | 299±6 |
tet-O7-TRR1 | Cytoplasmic thioredoxin reductase | 300±4 |
tet-O7-GPI16 | Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex | 307±6 |
tet-O7-NUP159 | Nucleoporin subunit of the nuclear pore complex | 310±10 |
tet-O7-NAB3 | Single-stranded RNA binding protein | 310±3 |