Representative western blot analysis of components of the non-neuronal cholinergic system from normal and FIC esophageal mucosa tissue extracts. A, Expression of enzymes involved in acetylcholine synthesis (choline acetyltransferase, ChAT; carnitine acetyltransferase, CarNT) and degradation (acetylcholinesterase, AChE). B, Expression of molecular transporters including choline transporter (CHT), members of the organic cationic transporter family (OCT-1, OCT-2, OCT3), the vesicular acetylcholine transporter (VAChT) and VNUT, a transporter responsible for filling vesicles with ATP. C, Expression of pan-cadherin, an adhesion molecule, and muscarinic receptor type 2 (M2) and type 3 (M3). Shown in each panel is the loading control β-actin.