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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Neurochem. 2012 May 30;122(2):333–343. doi: 10.1111/j.1471-4159.2012.07780.x

Figure 3. Neuroprotective effect of RGS10 in DA cells is associated with enhanced phospho-CREB signaling and increased levels of the pro-survival protein Bcl-2.

Figure 3

Neurally differentiated parental MN9D cells or cell lines stably expressing WT or SA mutant RGS10 were treated with 10 ng/mL of TNF for 0, 5 min, 15 min, 1, or 4 hrs. A, Levels of phospho-CREB and total CREB by western blot analysis. Densitometry and normalization to GAPDH as a protein loading control was performed. Blots are representative of 3 independent experiments. B, Relative levels of phospho-CREB in parental MN9D cells or cell lines stably expressing WT or SA mutant RGS10 under control or TNF (10 ng/mL) -stimulated conditions. Values represent mean ± SEM. One way ANOVA followed by Tukey’s post hoc test, *, **, and *** denote significant differences between the groups indicated at p < 0.05, p < 0.01, or p < 0.001 respectively. C, Levels of Bcl-2 protein by western blot on parental or stably expressing WT RGS10 or SA mutant RGS10 MN9D cells treated with TNF (10 ng/mL) for 0, 1, 4 or 6 hrs. Densitometry and normalization to α-tubulin as a protein loading control was performed. Blots are representative of three independent experiments.