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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Neurochem. 2012 May 30;122(2):333–343. doi: 10.1111/j.1471-4159.2012.07780.x

Figure 5. Neuroprotective effect of RGS10 in DA cells does not require modulation of p38 MAPK signaling.

Figure 5

A, Levels of phospho-p38MAPK and total p38 MAPK upon TNF-treatments. Neurally differentiated parental MN9D cells or cell lines stably expressing WT or SA mutant RGS10 were treated with 10 ng/mL of TNF for 0, 15 min, 1 or 4 hrs as indicated. Densitometry and normalization to α-tubulin as a protein loading control was performed. Blots are representative of three independent experiments. B, Effect of p38 inhibition on TNF-induced cytotoxicity. Neurally differentiated MN9D cells were treated with TNF (10 ng/mL) for 48 hrs in the presence or absence of pretreatment of SB203580 (10 µM) for 45 min. Cell viability was assessed using the MTS assay. Values represent mean ± SEM. One way ANOVA followed by Tukey’s post hoc test, *, ** or *** denote significant differences between vehicle and TNF treatments within the group at p < 0.05, p < 0.01 or p < 0.001 respectively.