Table 1. Zebrafish T-ALL is molecularly similar to mouse and human disease.

Microarray gene expression signatures were identified by comparing zebrafish T-ALL to normal thymocytes. The up- and down-regulated gene sets were assessed for concordant gene regulation in human and mouse disease. The mouse T-ALL to thymocyte comparisons were made using GSE19499 and GSE7050 data sets, while human T-ALL to B-ALL comparisons were made using GSE13425. Similar results were observed using the human GSE13351 data set (data not shown). GSEA significance was defined by p-value <0.05 and a false-discovery rate of ≤0.25 (FDR) at three different fold cut-offs. Number of common probes found at each fold-change cut off (# of probes), Enrichment Score (ES), Normalized Enrichment Score (NES). Negative ES and NES scores indicate that the gene set is down regulated within the samples being compared.

GSEA Comparison	Gene Set	# of probes	ES	NES	FDR	p-value
Mouse T-ALL to thymocytes	2.5 fold Up	585	0.486	2.031	0.000	<0.001
Mouse T-ALL to thymocytes	3.0 fold Up	361	0.428	1.841	0.000	<0.001
Mouse T-ALL to thymocytes	3.5 fold Up	192	0.404	1.699	0.002	0.001
Mouse T-ALL to thymocytes	2.5 fold Down	532	−0.367	−1.770	0.000	<0.001
Mouse T-ALL to thymocytes	3.0 fold Down	348	−0.361	−1.584	0.009	0.004
Mouse T-ALL to thymocytes	3.5 fold Down	199	−0.385	−1.579	0.007	0.003
Human T-ALL to B-ALL	2.5 fold Up	534	0.465	2.077	0.000	<0.001
Human T-ALL to B-ALL	3.0 fold Up	312	0.468	2.001	0.000	<0.001
Human T-ALL to B-ALL	3.5 fold Up	156	0.442	1.773	0.000	<0.001
Human T-ALL to B-ALL	2.5 fold Down	517	−0.355	−1.637	0.000	<0.001
Human T-ALL to B-ALL	3.0 fold Down	328	−0.395	−1.752	0.000	<0.001
Human T-ALL to B-ALL	3.5 fold Down	209	−0.426	−1.799	0.000	<0.001