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. Author manuscript; available in PMC: 2013 Oct 18.
Published in final edited form as: Oncogene. 2012 Jun 4;32(16):2079–2086. doi: 10.1038/onc.2012.221

Figure 4. Overexpression of exogenous ERRs increases glycolytic gene expression and aerobic glycolysis in cancer cells.

Figure 4

(A) Immunoblotting analysis of ERRs and glycolytic enzymes ENO1 and LDHA in MCF7 cells transduced with control (“C”), or Flag-ERR-expressing lentivirus. Tubulin serves as a loading control. The ERRγ antibody only recognized exogenous protein. Arrowhead denotes a non-specific band (NS).

(B) Quantitative determination of glycolytic gene expression by RT-PCR in control, ERRα- and ERRγ-overexpressed MCF7 cells. * represents significant changes (p<0.05).

(C) Comparison of lactate levels in culture media from control, ERRα- and ERRγ-expressing MCF7 cells. * represents significant changes (p<0.05).